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Disadvantages & Advantages of an TLC

Submitted by frndzzz on Sat, 06/28/2014 - 15:00

Some Disadvantages & Advantages of an TLC.

In Thin layer Chromatography also called (TLC) is widely used method for the separation and detection of an molecule from a given mixture of compounds.

This method is used for the separation of non volatile compounds.

This is generally performed on the thin sheet of aluminum or thin sheet of glass or plastic.

This sheet are coated with different media, commonly used are silica gel, cellulose etc.

The sample is applied on this plate and it is called stationary phase.

Now the plate is dipped into the solvent or a mixture of solvents also called as mobile phase.Now the solvent will rise and the different components present in the sample will separate out .

Advantages

  • Very less equipment are used. It is very simple method. It is also sensitive method.
  • The components are separated in very little time as the components will elute out very quickly.
  • The components present in the sample can be separated and recovered  out easily by scratching the powdery coating on the plate  and hence quantitative separation of spots or zone are possible.
  • It is cheaper chromatographic methods compared to other 
  • Simple process with short development time
  • TLC helps in isolation of most of the compounds
  • TLC helps in visualization of separated compound spots easily
  • The purity standards of the given sample in TLC can be assessed easily
  • Any quantity of mixture can be separated
  • Any time of mixture can be separated
  • Automation is possible
  • Greater speed of separation
  • Wide choice of materials 
  • Multiple analysis possible
  • Easy to use and cheap
  • Small volumes
  • No sample preparation required

Disadvantages

  • In this method the plate length is limited and hence separation takes place only up to certain length.
  • The separation takes place in an open system or in open condition and hence there are chances that sample may be affected by the humidity and temperature.
  • Time consuming
  • More amount of mobile phase are required
  • Automation makes the process more expensive and complicated
  • TLC plates do not have long stationary phases
  • Limited quality of separation
  • Evaporation of the mobile phase
  • Limited reproducibility

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Disadvantages & Advantages of an GC

Submitted by frndzzz on Sat, 06/28/2014 - 14:14

Disadvantages & Advantages of an GC.

Gas chromatography (GC) is an analytical method which is used for the separation of an volatile substance from a give mixture of compounds which are very difficult to separate and analyse.

This type of chromatography separates the molecules based on the volatility of a substance.

The substance with more volatility will separate out quickly while the substance with less volatility will elute out slowly.

Advantages 

Advantages and disadvantages of Chromatography

Submitted by frndzzz on Sat, 06/28/2014 - 17:12

Chromatography is simple method. Chromatography can be handled by single person.

Other advantages are, Chromatography is used to separate components from complex mixture.

Different types of chromatography equipment are available for separation of different compounds.

Some of them are Gas Chromatography, Liquid Chromatography, Solid Chromatography, High performance liquid Chromatography, Solid liquid Chromatography, Liquid liquid Chromatography, Thin layer Chromatography. This method gives accurate results.

Advantages and disadvantages of solid phase extraction

Submitted by frndzzz on Sun, 06/29/2014 - 12:38

Solid phase extraction:Let us discuss advantages and disadvantages of solid phase extraction. Some of them are discussed below so let us check it out one by one.

Advantages:

No emulsion problems observed.

Cost is low

Accuracy is greater

Glass breakage minimal

Lower solvent consumption

Reagent consumption lower

No cross contamination

Less sample handling

Process in fewer steps

Disadvantages:

Incomplete removal of the interferences

Types of chromatography

Submitted by frndzzz on Sun, 06/29/2014 - 15:52

There are various different types of chromatography which are used today in different industries and in day to day life.

Before going into detail let us check it out the definition of the chromatography.

The chromatogaphy is the separation of a component from of complex mixture of substance.

Types of component, functional group or structure can be identified with the help of chromatography.

List of some of the equipment are as follows:

Gas chromatography

Liquid chromatography

Solid chromatography

Liquid-liquid chromatography