Some Disadvantages & Advantages of an TLC.
In Thin layer Chromatography also called (TLC) is widely used method for the separation and detection of an molecule from a given mixture of compounds.
This method is used for the separation of non volatile compounds.
This is generally performed on the thin sheet of aluminium or thin sheet of glass or plastic.
This sheet are coated with different media, commonly used are silica gel, cellulose etc.
The sample is applied on this plate and it is called stationary phase.
Now the plate is dipped into the solvent or a mixture of solents also called as mobile phase.Now the solvent will rise and the different components present in the sample will separate out .
Very less equipment are used. It is very simple method. It is also sensitive method.
The components are separated in very little time as the components will elute out very quickly.
The components present in the sample can be separated and recovered out easily by scratching the powdery coating on the plate and hence quantitative separation of spots or zone are possible.
In this method the plate length is limited and hence separation takes place only upto certain length.
The separation takes place in an open system or in open condition and hence there are chances that sample may be affected by the humidity and temperature.