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Disadvantages & Advantages of an TLC

Some Disadvantages & Advantages of an TLC.

In Thin layer Chromatography also called (TLC) is widely used method for the separation and detection of an molecule from a given mixture of compounds.

This method is used for the separation of non volatile compounds.

This is generally performed on the thin sheet of aluminium or thin sheet of glass or plastic.

This sheet are coated with different media, commonly used are silica gel, cellulose etc.

The sample is applied on this plate and it is called stationary phase.

Now the plate is dipped into the solvent or a mixture of solents also called as mobile phase.Now the solvent will rise and the different components present in the sample will separate out .

Advantages

Very less equipment are used. It is very simple method. It is also sensitive method.

The components are separated in very little time as the components will elute out very quickly.

The components present in the sample can be separated and recovered  out easily by scratching the powdery coating on the plate  and hence quantitative separation of spots or zone are possible.

Disadvantages

In this method the plate length is limited and hence separation takes place only upto certain length.

The separation takes place in an open system or in open condition and hence there are chances that sample may be affected by the humidity and temperature.

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Disadvantages & Advantages of an GC

Disadvantages & Advantages of an GC.

Gas chromatography (GC) is an analytical method which is used for the separation of an volatile substance from a give mixture of compounds which are very difficult to separate and analyse.

This type of chromatography separates the molecules based on the volatility of a substance.

The substance with more volatility will separate out quickly while the substance with less volatility will elute out slowly.

Advantages 

Advantages and Disadvantages of International Trade

Check out Advantages and Disadvantages of International Trade discussed below. So let us find out some information on advantages and disadvantages to know more about International Trade. It is a trade between nations in the form of imports as well as exports. International trade can be considered one of the important souce of revenue in the developing countries. Trade between countries is the exchage of both services and goods among the countries of the world economy.