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What happens in Chromatography?

What happens in Chromatography? Let us check it out how chromatography works and what is the principle behind chromatography. Principle of chromatography is given below.

  1. Chromatography is a method or technique used to separate or isolate different constituents of a solution mixture.
  2. Chromatography separates unknown sample from a give mixture.
  3. Compound which is to be separated is passed through chromatography column along with the mobile phase.
  4. There is stationary phase in the chromatography column.
  5. Now the molecules of given compouds interacts (adsorb) with the stationary phase.
  6. Molecules which have strong interaction will separate slower and molecules with weak interaction will move fast.
  7. The molecules of given compound must have solubility properties with the mobile phase.
  8. Finally molecules are detected at the detector and hence unknown molecule can be identified.

 

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Types of chromatography

There are various different types of chromatography which are used today in different industries and in day to day life.

Before going into detail let us check it out the definition of the chromatography.

The chromatogaphy is the separation of a component from of complex mixture of substance.

Types of component, functional group or structure can be identified with the help of chromatography.

List of some of the equipment are as follows:

Gas chromatography

Liquid chromatography

Solid chromatography

Liquid-liquid chromatography

Advantages and disadvantages of Chromatography

Chromatography is simple method. Chromatography can be handled by single person.

Other advantages are, Chromatography is used to separate components from complex mixture.

Different types of chromatography equipment are available for separation of different compounds.

Some of them are Gas Chromatography, Liquid Chromatography, Solid Chromatography, High performance liquid Chromatography, Solid liquid Chromatography, Liquid liquid Chromatography, Thin layer Chromatography. This method gives accurate results.

Full Form of NPC Chromatography

Normal Phase Chromatography (NPC) is an analytical method used to separate substance from a given mixture. (Other full form of NPC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.

Full Form of TLC Chromatography

Thin Layer Chromatography (TLC) is an analytical method used to separate substance from a given mixture. (Other full form of TLC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.

Full Form of GC Chromatography

Gas Chromatography (GC) is an analytical method used to separate substance from a given mixture. (Other full form of GC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.

Full Form of HPLC Chromatography

High Performance Liquid Chromatography (HPLC) is an analytical method used to separate substance from a given mixture. (Other full form of HPLC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.

Disadvantages & Advantages of an GC

Disadvantages & Advantages of an GC.

Gas chromatography (GC) is an analytical method which is used for the separation of an volatile substance from a give mixture of compounds which are very difficult to separate and analyse.

This type of chromatography separates the molecules based on the volatility of a substance.

The substance with more volatility will separate out quickly while the substance with less volatility will elute out slowly.

Advantages 

Advantages and disadvantages of solid phase extraction

Solid phase extraction:Let us discuss advantages and disadvantages of solid phase extraction. Some of them are discussed below so let us check it out one by one.

Advantages:

No emulsion problems observed.

Cost is low

Accuracy is greater

Glass breakage minimal

Lower solvent consumption

Reagent consumption lower

No cross contamination

Less sample handling

Process in fewer steps

Disadvantages:

Incomplete removal of the interferences