You are here

What Happens In Paper Chromatography?

What happens in Paper Chromatography? - Let us see the principle of paper chromatography, How does Paper Chromatography works. Principle of paper chromatography is given below.

  1. Paper chromatography is used to separate coloured chemicals.
  2. Paper chromatography is used to separate organic as well as inorganic compounds.
  3. Mobile phase and stationary phase are present in paper chromatography.
  4. Solid or liquid supported on solid is stationary phase.
  5. Mobile phase may be liquid or a gas.
  6. The mobile phase flows on the stationary phase and carries the component mixture with it.
  7. Different components travel at different rates.
  8. Rf value is calculated and the given unknown component is identified.
  9. Paper chromatography is useful in Biochemicals, Determination of hormones, Drugs etc.

Related article : Advantages & Disadvantages of Paper Chromatography

Explore more Information

Types of chromatography

There are various different types of chromatography which are used today in different industries and in day to day life.

Before going into detail let us check it out the definition of the chromatography.

The chromatogaphy is the separation of a component from of complex mixture of substance.

Types of component, functional group or structure can be identified with the help of chromatography.

List of some of the equipment are as follows:

Gas chromatography

Liquid chromatography

Solid chromatography

Liquid-liquid chromatography

Advantages and disadvantages of Chromatography

Chromatography is simple method. Chromatography can be handled by single person.

Other advantages are, Chromatography is used to separate components from complex mixture.

Different types of chromatography equipment are available for separation of different compounds.

Some of them are Gas Chromatography, Liquid Chromatography, Solid Chromatography, High performance liquid Chromatography, Solid liquid Chromatography, Liquid liquid Chromatography, Thin layer Chromatography. This method gives accurate results.

Full Form of NPC Chromatography

Normal Phase Chromatography (NPC) is an analytical method used to separate substance from a given mixture. (Other full form of NPC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.

Advantages and disadvantages of solid phase extraction

Solid phase extraction:Let us discuss advantages and disadvantages of solid phase extraction. Some of them are discussed below so let us check it out one by one.

Advantages:

No emulsion problems observed.

Cost is low

Accuracy is greater

Glass breakage minimal

Lower solvent consumption

Reagent consumption lower

No cross contamination

Less sample handling

Process in fewer steps

Disadvantages:

Incomplete removal of the interferences

Full Form of HPLC Chromatography

High Performance Liquid Chromatography (HPLC) is an analytical method used to separate substance from a given mixture. (Other full form of HPLC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.

Full Form of TLC Chromatography

Thin Layer Chromatography (TLC) is an analytical method used to separate substance from a given mixture. (Other full form of TLC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.

Full Form of GC Chromatography

Gas Chromatography (GC) is an analytical method used to separate substance from a given mixture. (Other full form of GC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.

Disadvantages & Advantages of an TLC

Some Disadvantages & Advantages of an TLC.

In Thin layer Chromatography also called (TLC) is widely used method for the separation and detection of an molecule from a given mixture of compounds.

This method is used for the separation of non volatile compounds.

This is generally performed on the thin sheet of aluminium or thin sheet of glass or plastic.

This sheet are coated with different media, commonly used are silica gel, cellulose etc.

The sample is applied on this plate and it is called stationary phase.