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Advantages And Disadvantages Of Affinity Chromatography

Advantages and Disadvantages of Affinity Chromatography - Check out more information of advantages and disadvantages of affinity chromatography to know more about chromatography.

Some of the Advantages of Affinity Chromatography are:

  1. Affinity Chromatography is used to study enzymes and other proteins.
  2. Affinity chromatography is used in genetic engineering.
  3. Also used during production of vaccines.
  4. Protein or Enzyme purification by affinity chromatography.
  5. Very high degree of purity can be seen with affinity chromatography.
  6. High specificity with affinity chromatography.

Some of the Disadvantages of Affinity Chromatography are:

  1. Ligands used in affinity chromatography are expensive.
  2. Leakage of ligands is also observed sometimes.
  3. Low productivity with affinity chromatography.
  4. Non-specific adsorption in affinity chromatography.

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Types of chromatography

There are various different types of chromatography which are used today in different industries and in day to day life.

Before going into detail let us check it out the definition of the chromatography.

The chromatogaphy is the separation of a component from of complex mixture of substance.

Types of component, functional group or structure can be identified with the help of chromatography.

List of some of the equipment are as follows:

Gas chromatography

Liquid chromatography

Solid chromatography

Liquid-liquid chromatography

Advantages and disadvantages of Chromatography

Chromatography is simple method. Chromatography can be handled by single person.

Other advantages are, Chromatography is used to separate components from complex mixture.

Different types of chromatography equipment are available for separation of different compounds.

Some of them are Gas Chromatography, Liquid Chromatography, Solid Chromatography, High performance liquid Chromatography, Solid liquid Chromatography, Liquid liquid Chromatography, Thin layer Chromatography. This method gives accurate results.

Full Form of HPLC Chromatography

High Performance Liquid Chromatography (HPLC) is an analytical method used to separate substance from a given mixture. (Other full form of HPLC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.

Full Form of TLC Chromatography

Thin Layer Chromatography (TLC) is an analytical method used to separate substance from a given mixture. (Other full form of TLC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.

Full Form of NPC Chromatography

Normal Phase Chromatography (NPC) is an analytical method used to separate substance from a given mixture. (Other full form of NPC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.

Full Form of GC Chromatography

Gas Chromatography (GC) is an analytical method used to separate substance from a given mixture. (Other full form of GC and related terms are also available) So check it out similar articles related to the given term.

Meaning and full form of other types of chromatography in chemistry are also available.

Disadvantages & Advantages of an GC

Disadvantages & Advantages of an GC.

Gas chromatography (GC) is an analytical method which is used for the separation of an volatile substance from a give mixture of compounds which are very difficult to separate and analyse.

This type of chromatography separates the molecules based on the volatility of a substance.

The substance with more volatility will separate out quickly while the substance with less volatility will elute out slowly.

Advantages